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KMID : 0861020080230030155
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Korea Journal of Herbology
2008 Volume.23 No. 3 p.155 ~ p.162
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Inhibitory Effect of Bodusan on TNF-α-induced Migration via Inhibition of Metalloproteinase Activity in Human Aortic Smooth Muscle Cells
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Yun Hyun-Jeong
Park Sun-Dong
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Abstract
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Objectives : This study was evaluated to elucidate the inhibitory potential of Bodusan (BDS) and its components, Strychnos ignatii semen (SIS) and Glycyrrhizae Radix (GR), on human aortic smooth muscle cells (HASMC) migration and production of matrix metalloproteinase (MMP)-2 and MMP-9 by TNF-α treatment.
Methods : Cytotoxic activity of BDS and its components on HASMC was using 5-(3-caroboxymethoxyphenyl)-
2H-tetra-zolium inner salt (MTS) assay. Effect of BDS, SIS and GR on TNF-α-induced HASMC migration underside of matrigel filter was stained with hematoxylin-eosin. And total number of cells that migrated to the underside of the filter was counted. MMP-2 and MMP-9 activity was evaluated by gelatin zymography assay.
Results : The matrigel migration assay showed that BDS effectively inhibited the TNF-α-induced migration of HASMC. Moreover, BDS significantly inhibited MMP-9 activity. Our present study demonstrates that BDS and its components inhibits TNF-α-induced HASMC migration and MMP-9 activity. The inhibitory effect of BDS extract is more potent than that of its component herb extracts.
Conclusions : These results provide evidence that BDS has multiple effects in the inhibition of HASMC migration and may offer a therapeutic approach to block HASMC migration.
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KEYWORD
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Bo-du-san, BDS, Bo-Du (SIS), Gam-Cho (GR), migration, matrix metalloproteinase, MMP
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